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Product Info Summary
| SKU: | A12249 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-AP-3 complex subunit sigma-1 AP3S1 Antibody
SKU/Catalog Number
A12249
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-AP-3 complex subunit sigma-1 AP3S1 Antibody (Catalog # A12249). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
AP3S1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-AP-3 complex subunit sigma-1 AP3S1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12249)
Host
Rabbit
Contents
AP3S1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
AP3S1 antibody was raised against a 19 amino acid synthetic peptide near the center of human AP3S1. The immunogen is located within the first 50 amino acids of AP3S1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of AP3S1 are known to exist; this antibody will detect the two larger isoforms. AP3S1 antibody is predicted to not cross-react with other AP3 protein family members.
Reactive Species
A12249 is reactive to AP3S1 in Human, Mouse, Rat
Applications
A12249 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
21.732kDa
Background of AP3S1
AP3S1 belongs to the adaptor complexes 3 (AP3) small subunit family which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. AP3 is an heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP3S1 Interacts with AGAP1 and may play an important role in carcinoma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
AP3S1 antibody can be used for detection of AP3S1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of AP3S1 in mouse kidney tissue lysate with AP3S1 antibody at (A) 1 and (B) 2 μg/mL .
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Immunohistochemistry of AP3S1 in human kidney tissue with AP3S1 antibody at 2.5 μg/mL.
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Immunofluorescence of AP3S1 in human kidney tissue with AP3S1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For AP3S1 (Source: Uniprot.org, NCBI)
Gene Name
AP3S1
Full Name
AP-3 complex subunit sigma-1
Weight
21.732kDa
Superfamily
adaptor complexes small subunit family
Alternative Names
Adapter-related protein complex 3 sigma-1 subunit; adaptor-related protein complex 3, sigma 1 subunit; AP-3 complex sigma-3A subunit; AP-3 complex subunit sigma-3A; CLAPS3AP-3 complex subunit sigma-1; Clathrin-associated/assembly/adapter protein, small 3; clathrin-associated/assembly/adaptor protein, small 3 (22kD); Sigma3A; sigma-3A-adaptin; sigma3A-adaptin; Sigma-adaptin 3a AP3S1 CLAPS3, Sigma3A adaptor related protein complex 3 subunit sigma 1 AP-3 complex subunit sigma-1|adapter-related protein complex 3 subunit sigma-1|adaptor related protein complex 3 sigma 1 subunit|clathrin adaptor complex AP3, sigma-3A subunit|clathrin-associated/assembly/adapter protein, small 3|clathrin-associated/assembly/adaptor protein, small 3 (22kD)|sigma-adaptin 3a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AP3S1, check out the AP3S1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AP3S1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AP-3 complex subunit sigma-1 AP3S1 Antibody (A12249)
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